egg protein calculator
Calculate Protein Solubility
About Protein Solubility
Protein solubility is influenced by various factors including pH, ionic strength, temperature, and the protein's physicochemical properties.
pH Effect:
Proteins are least soluble at their isoelectric point (pI) and more soluble away from pI
Salt Effect:
Low salt concentrations increase solubility (salting-in), high concentrations decrease solubility (salting-out)
Temperature Effect:
Most proteins become less soluble at higher temperatures due to denaturation
Key factors affecting solubility:
- pH relative to isoelectric point
- Ionic strength of solution
- Temperature
- Protein size and charge
- Presence of denaturants
Understanding protein solubility is crucial in protein purification, formulation, and biotechnology applications.