Enzyme Activity Calculator

Free calculate enzyme activity, michaelis-menten kinetics, vmax, km, and enzyme inhibition parameters.

Enter Enzyme Data

Optional - for inhibition studies

Results

Enter enzyme data to calculate activity

Michaelis-Menten Equation

Michaelis-Menten Equation

v = (Vmax × [S]) / (Km + [S])

  • • v = Initial velocity
  • • Vmax = Maximum velocity
  • • [S] = Substrate concentration
  • • Km = Michaelis constant
  • • Km = [S] when v = Vmax/2

Enzyme Parameters

  • • Vmax: Maximum reaction rate
  • • Km: Substrate affinity
  • • kcat: Turnover number
  • • kcat/Km: Catalytic efficiency
  • • Lower Km = higher affinity

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