Beer-Lambert Law Calculator

Calculate absorbance, concentration, molar absorptivity, and path length using the Beer-Lambert law.

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Beer-Lambert Law

Mathematical Form

A = ε × c × l

  • • A = Absorbance (dimensionless)
  • • ε = Molar absorptivity (L/mol·cm)
  • • c = Concentration (mol/L)
  • • l = Path length (cm)

Applications

  • • UV-Vis spectrophotometry
  • • Concentration determination
  • • Purity analysis
  • • Reaction kinetics
  • • Quality control

Common Molar Absorptivity Values

Organic Compounds

  • • Benzene: ~200 L/mol·cm
  • • Naphthalene: ~10,000 L/mol·cm
  • • Anthracene: ~100,000 L/mol·cm

Metal Complexes

  • • Fe(SCN)²⁺: ~4,000 L/mol·cm
  • • Cu(NH₃)₄²⁺: ~60 L/mol·cm
  • • MnO₄⁻: ~2,200 L/mol·cm

Biomolecules

  • • DNA (260 nm): ~6,600 L/mol·cm
  • • Protein (280 nm): ~1,000 L/mol·cm
  • • Hemoglobin: ~125,000 L/mol·cm