qPCR Efficiency Calculator

Calculate qPCR amplification efficiency, R², slope, and validation parameters for quantitative PCR analysis.

qPCR Data Input

Results

Enter qPCR data to calculate efficiency

qPCR Efficiency Guidelines

Efficiency Standards

  • • 90-110%: Excellent
  • • 85-95%: Good
  • • 80-90%: Acceptable
  • • <80%: Poor
  • • >110%: Inhibited

R² Requirements

  • • >0.99: Excellent
  • • 0.98-0.99: Good
  • • 0.95-0.98: Acceptable
  • • <0.95: Poor
  • • Minimum: 0.95

Slope Values

  • • -3.1 to -3.6: Ideal
  • • -3.0 to -3.8: Acceptable
  • • -2.5 to -4.0: Marginal
  • • <-2.5: Poor
  • • >-4.0: Inhibited