Protein Concentration Calculator
Calculate protein concentration using Bradford, Lowry, and BCA assay methods with standard curves.
Protein Assay Parameters
Results
Enter assay parameters to calculate protein concentration
Protein Assay Methods Guide
Bradford Assay
- • Range: 1-1400 μg/mL
- • Wavelength: 595 nm
- • Fast and simple
- • Compatible with detergents
- • Sensitive to pH changes
Lowry Assay
- • Range: 1-1500 μg/mL
- • Wavelength: 750 nm
- • More sensitive than Bradford
- • Affected by detergents
- • Requires multiple steps
BCA Assay
- • Range: 20-2000 μg/mL
- • Wavelength: 562 nm
- • Compatible with detergents
- • Stable color development
- • Less sensitive to pH