DNA Concentration Calculator
Calculate DNA concentration from absorbance readings using Beer's Law and determine DNA purity ratios.
Spectrophotometer Readings
Enter 1 if no dilution, or the dilution factor used
Results
Enter absorbance readings to calculate concentration
DNA Concentration Calculations
Extinction Coefficients
- • dsDNA: 50 μg/mL per A260 unit
- • ssDNA: 33 μg/mL per A260 unit
- • RNA: 40 μg/mL per A260 unit
- • Formula: C = A260 × ε × DF
Purity Ratios
- • A260/A280: 1.8-2.0 (pure DNA)
- • A260/A230: >2.0 (clean DNA)
- • Lower ratios indicate contamination
- • Protein contamination lowers A260/A280